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A-Level Biology (9700) Specification

The Cambridge International AS & A Level Biology syllabus (9700): 5 papers, 19 topics, three assessment objectives (AOs), and the practical papers.

Source: CIE, v2025-2027 · Reviewed by Emily Clarke · Last reviewed 2026-08-05

specification overview

Cambridge International A Level Biology (9700) is a two-year linear qualification covering 19 topics from cell structure through to genetic technology, sat as five papers. It's graded A*-E (or a-e for AS Level only), with two dedicated practical papers (Paper 3 hands-on, Paper 5 planning and analysis) alongside three theory papers.

specification overview

subject content

  1. 1. Cell structure

    Paper 1, Paper 2
    • The microscope in cell studies
    • Cells as the basic units of living organisms
    • Prokaryotic and eukaryotic cell structure
  2. 2. Biological molecules

    Paper 1, Paper 2
    • Testing for biological molecules
    • Carbohydrates and lipids
    • Proteins and water
  3. 3. Enzymes

    Paper 1, Paper 2
    • Mode of action of enzymes
    • Factors affecting enzyme action
  4. 4. Cell membranes and transport

    Paper 1, Paper 2
    • Fluid mosaic membranes
    • Movement into and out of cells (diffusion, osmosis, active transport)
  5. 5. The mitotic cell cycle

    Paper 1, Paper 2
    • Replication and division of nuclei and cells
    • Chromosome behaviour in mitosis
  6. 6. Nucleic acids and protein synthesis

    Paper 1, Paper 2
    • Structure and replication of DNA
    • Protein synthesis
  7. 7. Transport in plants

    Paper 1, Paper 2
    • Structure of transport tissues
    • Transport mechanisms (xylem, phloem, translocation)
  8. 8. Transport in mammals

    Paper 1, Paper 2
    • The circulatory system
    • The heart
    • Blood and tissue fluid
  9. 9. Gas exchange

    Paper 1, Paper 2
    • The gas exchange system
  10. 10. Infectious diseases

    Paper 1, Paper 2
    • Infectious diseases
    • Antibiotics
  11. 11. Immunity

    Paper 1, Paper 2
    • The immune system
    • Antibodies and vaccination
  12. 12. Energy and respiration

    Paper 4
    • Energy
    • Respiration
  13. 13. Photosynthesis

    Paper 4
    • Photosynthesis as an energy transfer process
    • Investigation of limiting factors
    • Adaptations for photosynthesis
  14. 14. Homeostasis

    Paper 4
    • Homeostasis in mammals
    • Homeostasis in plants
  15. 15. Control and coordination

    Paper 4
    • Control and coordination in mammals
    • Control and coordination in plants
  16. 16. Inheritance

    Paper 4
    • Passage of information from parents to offspring
    • The roles of genes in determining the phenotype
    • Gene control
  17. 17. Selection and evolution

    Paper 4
    • Variation
    • Natural and artificial selection
    • Evolution
  18. 18. Classification, biodiversity and conservation

    Paper 4
    • Classification
    • Biodiversity
    • Conservation
  19. 19. Genetic technology

    Paper 4
    • Principles of genetic technology
    • Genetic technology applied to medicine
    • Genetically modified organisms in agriculture
subject content

assessment structure

PaperPaper 1
What's assessedMultiple choice on AS Level content (topics 1-11)
Length1h 15m
Marks40
Weight15.5%
Question types40 multiple-choice questions
PaperPaper 2
What's assessedStructured questions on AS Level content (topics 1-11)
Length1h 15m
Marks60
Weight23%
Question typesShort-answer and structured questions
PaperPaper 3
What's assessedAdvanced Practical Skills. Practical work and structured questions based on practical skills; contexts may fall outside the syllabus content
Length2h
Marks40
Weight11.5%
Question typesPractical work with structured questions
PaperPaper 4
What's assessedA Level Structured Questions on A Level content (topics 12-19); knowledge of AS content is assumed
Length2h
Marks100
Weight38.5%
Question typesStructured questions
PaperPaper 5
What's assessedPlanning, Analysis and Evaluation. Practical-skills questions on planning experiments, analysing data and evaluating methods; contexts may fall outside the syllabus content
Length1h 15m
Marks30
Weight11.5%
Question typesStructured questions on planning, analysis and evaluation

The full A Level route is all five papers. Students can also stage the qualification: Papers 1-3 in year 1 for the standalone AS Level (grades a-e), then Papers 4-5 in year 2 to complete the A Level.

assessment structure

assessment objectives

AOAO1
What it meansKnowledge and understanding of scientific phenomena, facts, laws, concepts and applications.
Overall40%
AOAO2
What it meansHandling, applying and evaluating information, including translating between forms, manipulating data, drawing conclusions and applying knowledge to new situations.
Overall40%
AOAO3
What it meansExperimental skills and investigations: planning, recording, analysing and evaluating experimental methods and data.
Overall20%
assessment objectives

key tips for success

  • Take Papers 3 and 5 seriously

    The practical papers are worth 23% of the A Level (11.5% each) and test skills, not memorised methods. Practise across a range of biological contexts, including ones outside the syllabus content.

  • AS content is assumed on Paper 4

    Paper 4 covers topics 12-19 but assumes you still remember topics 1-11. Revise the AS material as part of your Paper 4 preparation, not just the newer A Level topics.

  • Master the multiple-choice paper first

    Paper 1 is 40 marks in 75 minutes: just under two minutes per question. Practise timing early so you're not caught out.

  • Build a data-handling toolkit

    AO2 is 40% of the qualification. Practise translating between graphs, tables and prose; manipulating numerical data; and drawing reasoned conclusions.

  • Use worked practical examples for Paper 5

    Paper 5 asks you to plan experiments and evaluate methods. Practise writing full method plans with clear variables, controls, replication and analysis before the exam.

  • Know which route you're on

    There are three routes: AS only, staged A Level over two years, or complete A Level in one series. Confirm with your school which route you're following so you prepare the right papers.

key tips for success

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