Microscopy - exam practice

Which statement about differential staining is not true?

Which of the following best describes a microscope with high resolution?

A prokaryotic cell that is 1 µm in diameter is magnified 50,000 times in an electron micrograph.

What is the diameter of the cell in the electron micrograph?

An eyepiece graticule has a scale with 100 divisions. A stage micrometer has a scale with 50 divisions, each of which is 0.040 mm apart.

Using a × 40 objective lens, the whole length of this stage micrometer scale lines up with 15 divisions of the eyepiece graticule.

What is the actual length of the 100 division scale of the eyepiece graticule?

A student was asked to use the scale bar shown to calculate the magnification of a cell on a photomicrograph.

Which method could the student use to calculate the magnification of the cell?

Which lengths are equivalent to 1 µm?

1 - 1,000 mm

2 - 0.001 nm

3 - 0.001 mm

4 - 1,000,000 nm

5 - 0.01 mm

6 - 1,000 nm

A culture of human cells had its cell surface membranes removed, releasing the cell contents.

This material became contaminated by bacteria.

The material was then centrifuged, separating out the various cell structures according to size and mass.

Which cell structure would be separated out along with the bacteria?

Pancreatic cells have a diameter of 35 µm. Red blood cells have a diameter of 7,000 nm.

Which statement is correct?

Which cell structure can be seen only with an electron microscope?

Plant cells are stained and then viewed through a light microscope.

Which structures would be clearly visible at a magnification of × 400?